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Protein scaffold in dna1/6/2024 Compared with MSCs from other sources, dental stem cells can be obtained more conveniently and are considered to be a promising candidate for tissue engineering and regenerative medicine.ĭental pulp stem cells (DPSCs), a type of odontogenic mesenchymal stem cell that originates from the transitional nerve crucible cells, are isolated from dental pulp tissue and have been demonstrated to have the ability to differentiate into odontoblasts, osteoblasts, chondroblasts, neuroblasts, fibroblasts, vascular cells, endothelial cells, pericyte-like cells, smooth muscle-like cells, and inner ear hair cells. MSCs can be isolated from a wide range of sources, such as bone marrow, peripheral blood, dental tissue, and the placenta. Mesenchymal stem cells (MSCs) have the potential for self-renewal and multi-directional differentiation thus, MSCs-based cellular therapies have been considered for the repair of damaged tissue. The tissue engineering technology, which utilizes the multi-differentiation potential capacity of stem cells, is expected to be used to repair damaged tissues or organs. Constructing a dentin/bone tissue regeneration therapy system has become a hot focus in the field of tissue engineering and regenerative medicine. Keywords: Dental pulp stem cells, Differentiation, LIM mineralization protein-1, Mitogen-activated protein kinase pathway, Tissue regeneration Introductionĭisease and trauma have resulted in a high incidence of dentin or alveolar bone defects, which severely affect the quality of life of patients. Our results not only indicated the underlying mechanism of LMP-1 treated DPSCs but also provided valuable insight into therapeutic strategies in regenerative medicine. Besides, the expression of BMP signaling pathway components were also determined, which suggested that LMP-1 could activate BMP-2/Smad1/5 signaling pathway. Furthermore, inhibiting the activation of the extracellular signal-regulated kinase 1/2 (ERK1/2), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) pathways using specific pathway inhibitors showed that the ERK1/2 and p38 MAPK pathways attenuated the differentiation of DPSCs. Overexpression of LMP-1 significantly promoted the committed differentiation of DPSCs and vice versa, as shown by alkaline phosphatase activity assay, alizarin red staining, western blot assay, quantitative real-time polymerase chain reaction assay, and in vivo mineralized tissue formation assay. As indicated by the cell counting kit-8 assay, the results showed that LMP-1 did not affect the proliferation of DPSCs. In this study, an LMP-1-induced DPSCs model was used to explore the effect of LMP-1 on the proliferation and odonto/osteogenic differentiation of DPSCs, as well as the underlying mechanisms. LIM mineralization protein-1 (LMP-1) is an intracellular non-secretory protein that plays a positive regulatory role in the mineralization process. Dental pulp stem cells (DPSCs) have been extensively studied for their use in tissue regeneration, including the regeneration of dentin and bone tissue. Tissue regeneration is the preferred treatment for dentin and bone tissue defects. Select the file that you have just downloaded and select import option Reference Manager (RIS). Available fromĬlick on Go to download the file. LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling. Mu R, Chen B, Bi B, Yu H, Liu J, Li J, He M, Rong L, Liu B, Liu K, Zhu L, Shi X, Shuai Y, Jin L. Yi Shuai, Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, School of Stomatology of Southern Medical University, Clinical Medical School of Nanjing Medical University, Nanjing 210002, China. ✉ Corresponding authors: Lei Jin, Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, School of Stomatology of Southern Medical University, Clinical Medical School of Nanjing Medical University, Nanjing 210002, China. Stomatology Center, Peking University Shenzhen Hospital, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Guangdong province, Shenzhen 518036, China. Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, School of Stomatology of Southern Medical University, Clinical Medical School of Nanjing Medical University, Nanjing 210002, China.Ģ. Rui Mu 1,2, Bo Chen 1, Bo Bi 1, Hongchuan Yu 1, Juan Liu 1, Junxia Li 1, Maodian He 1, Liang Rong 1, Bingyao Liu 1, Ke Liu 1, Lei Zhu 1, Xiaolei Shi 1, Yi Shuai 1, Lei Jin 1ġ. Research Paper LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling
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